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为探究酸枣HMGS(3-羟基-3-甲基戊二酰辅酶A合酶)基因和蛋白功能，基于全基因组数据，对ZjHMGS基因进行了鉴定、克隆及表达分析。结果表明，酸枣ZjHMGS全长CDS为1362 bp，编码454个氨基酸，分子量50.45 kDa;ZjHMGS定位于细胞质，是一种酸性不稳定亲水蛋白，且属于非分泌蛋白和非跨膜蛋白；系统发育分析表明HMGS基因可能起源于裸子植物与被子植物分化前；RT-qPCR显示ZjHMGS在酸枣叶中的表达最高。研究结果为后续深入研究酸枣皂苷生物合成提供了一定的理论依据。
Abstract：To investigate the gene and protein functions of Ziziphus jujuba var. spinosa HMGS(3-hydroxy-3-methylglutaryl coenzyme A synthase), the ZjHMGS gene was identified, cloned and analyzed for bioinformatics characteristics based on genome-wide data. The results demonstrated that ZjHMGS possesses a full-length CDS of 1362 bp, encodes 454 amino acids, and possesses a molecular weight of 50.45 kDa.ZjHMGS is localized in the cytoplasm, is an acidic, unstable, hydrophilic protein, and is a non-secretory and non-transmembrane protein. Phylogenetic analyses indicate that the HMGS gene may have originated before the differentiation of gymnosperms from angiosperms. RT-qPCR analysis revealed that the highest expression of ZjHMGS was observed in the leaves of jujube. The results provide a theoretical basis for the subsequent in-depth study of jujube saponin biosynthesis.
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基本信息:
DOI：10.15948/j.cnki.37-1500/s.2025.09.001
中图分类号:S665.1
引用信息:
[1]张玉才,赵梦楠,王信宏,等.酸枣ZjHMGS基因的克隆及表达分析[J].山东农业工程学院学报,2025,42(09):22-28.DOI:10.15948/j.cnki.37-1500/s.2025.09.001.
基金信息:
山东省中医药科技项目“黄河流域耐盐碱酸枣品种选育及规范化种植研究”（M2023049）; 济南市农业科技攻关项目“酸枣良种引进筛选及其集约化育苗”（GG202402）; 国家重点研发计划“地下灌溉对苹果水分利用效率及根系周年形态变化研究”（2019YFD1000102）; 山东省科技型中小企业创新能力提升工程项目“耐盐富硒饲用蒲公英高产栽培与高效利用关键技术研究和应用”（2023TSGC0281）